GenCRISPR™ LbuCas13a Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as LbuC2c2.

產(chǎn)品型號(hào)：Z03742

更新時(shí)間：2026-03-02

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分子生物學(xué)

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產(chǎn)品介紹

產(chǎn)品概述

Description

GenCRISPR™ LbuCas13a Nuclease is an RNA-guided RNA endonuclease from Leptotrichia buccalis. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system and contains a single protein effector with two HEPN domains. Cas13a exhibits two distinct ribonuclease activities. Cas13a possesses sequence-specific RNA cleavage activity in vitro and in vivo. And after specific cleavage, Cas13a activates collateral cleavage activity towards adjacent non-specific ssRNA sequences. The two distinct ribonuclease activities of Cas13a enable guide-RNA processing and RNA detection. The RNA detection empowered with Cas13a-crRNA complex and quenched fluorescent ssRNA as reporter has been reported to be versatile, rapid and high-specific.

應(yīng)用指導(dǎo)

crRNA-dependent RNA cleavage

產(chǎn)品屬性

Source	Recombinant Cas13a expressed by E.coli
Species	Leptotrichia buccalis
Tag	Tag-free
Apparent Molecular Weight	~140 kDa, on SDS-PAGE under non-reducing conditions
Concentration	4 mg/ml
Storage Buffer	50 mM Tris-HCI, 600 mM NaCl, 0.1 mM TCEP, 50% glycerol, pH 7.5.
Storage & Stability	Store at -20 °C for 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C!
Accession#	C7NBY4

質(zhì)控參數(shù)

Appearance	Clear, colorless liquid
Purity	≥ 90% by SDS-PAGE
Activity	≥ 85% by in vitro digestion of ssRNA
Concentration	4 mg/ml ± 10% as analyzed by A280
Residual DNase	Undetectable
Residual RNase	Undetectable
Endotoxin Level	< 0.2 EU/μg